Table 1. HPLC conditions for cyanocobalamin analysis

Items	HPLC condition
HPLC system	Agilent 1260 infinity (Agilent, Santa Clara, CA, USA)
Detection	Diode array detector (DAD, 361 nm)
Column	ACE 3 AQ (150 mm×3 mm, ACE, Aberdeen, Scotland, UK)
Mobile phase	A: Water, B: Acetonitrile
	Time (min)	A (%)	B (%)
	0	100	0
	11	85	15
	19	75	25
	20	90	10
	26	100	0
	40	100	0
Flow rate	0.25 mL/min
Injection volume	100 μL
Column oven temperature	35°C