Table 1. HPLC analytical conditions for maysin isolated from Eremochloa ophiuroides

Parameter	Condition
Instrument	Shimadzu LC-20AD
Detector	Shimadzu SPD-20A Prominence UV/VIS Detector
Wavelength	360 nm
Column	YMC-Pack C18 Column (4.6 mm I.D. × 150 mm; particle size 5 μm)
Oven temperature	40°C
Flow rate	1.0 mL/min
Injection volume	5 μL
Mobile phase	Time	A: 0.1% formic acid in H2O	B: acetonitrile
	5	85	15
	10	70	30
	15	50	50
	20	30	70
	25	0	100
	30	0	100
	31	95	5
	40	95	5