Table 1. Analytical conditions of LC-MS/MS for the determination of aflatoxins, ochratoxin A, zeralnenone and fumonisins

Instrument		Condition
HPLC	Column	C18 (Capcell core C18, 2.1×150 mm, 1.7 μm, Osaka soda)
	Column temperature	40°C
	Mobile phase	A: 5 mM ammonium formate, 0.1% formic acid in distilled water
		B: 5 mM ammonium formate, 0.1% formic acid in methanol
		Gradient
		Min	Flow rate	A (%)	B (%)
		0-0.5	0.4	95	5
		2	0.4	60	40
		9-11.5	0.4	0	100
		12-50	0.7	0	100
	Flow rate	0.4 mL/min
	Injection volume	20 μL
MS/MS	Ionization	ESI Positive, Negative mode
	Ion spray voltage	4,500
	Ion source temperature	500°C
	Collision energy (V)	9-30
	Ion source gas 1	50
	Ion source gas 2	50
	Gas	N2