Table 2. GC-MS operating conditions for analysis of eugenol (4-Allyl-2-methhoxyphenol)

Instrument	SHIMADZU GCMS QP 2020
Column	DB-35MS UI (30 m length, 0.25 mm diameter, 0.25 μm film thickness)
Temp.	Column temperature: 100°C (1 min) → 10°C/min, 300°C (4 min) Injection temperature: 250°C
Carrier gas	Helium
Gas flow rate	1.2 mL/min
Injection vol.	1.0 μL
Ion source temp.	250°C
Detection mode	Scan mode: 40-300 m/zSIM1) mode: 164, 149, 103, 131 m/z

SIM, selected ion monitoring.