Table S1. Single factor experiment design

Single factor	Variables levels
Time	60	120	180	240	300
Enzyme concentration (mg/mL)	0.5	1.5	2.5	3.5	4.5
pH	7	7.5	8	8.5	9
Substrate concentration (g/mL)	0.01	0.03	0.05	0.07	0.09