| HPLC | Column | Agilent Poroshell 120 EC-C18 column (1.9 μm, 2.1 × 50 mm) |
| Column oven temperature | 30°C |
| Injection volume | 3 μL |
| Flow rate | 0.3 mL/min |
| Mobile phase | A: Distilled waterB: Acetonitrile |
| Gradient Table | Time (min) | A (%) | B (%) |
| 0 | 95 | 5 |
| 4.5 | 50 | 50 |
| 6.5 | 50 | 50 |
| 6.51 | 95 | 5 |
| 9 | 95 | 5 |
| Instrument | Shimadzu LC/MS-8050 |
| MS/MS | Ionization mode | Electrospray ionization source, negative mode |
| Nebulizer gas flow | 3 L/mim |
| Heating, drying gas flow | 10 L/mim |
| Heat block temperature | 400°C |
| Interface temperature | 350°C |
| DL temperature | 100°C |
| MS/MS parameters |
| Compound1) | Quantifier ion (m/z) | Qualifier ion (m/z) |
| Lar | 359.45>329.15 | 359.45>160 |
| Mat | 357.45>83.05 | 357.45>122.05 |
| Med | 387.45>99.0 | 387.45>193 |
| Pin | 357.45>151.15 | 357.45>135.90 |
| Seco | 361.05>165.15 | 361.05>121.10 |
| Syr | 417.45>181.15 | 417.45>166.10 |