Table 2. Validation of analytical method for pantothenic acid

Vitamin	Sensitivity		Precision		Recovery and accuracy
	LOD¹⁾	LOQ²⁾	Repeatability³⁾	Reproducibility⁴⁾	Reference value⁶⁾	Analysis value⁷⁾	Recovery (%)	RSD (%)
	(μg/mL)		RSD (%)⁵⁾	RSD (%)	Reference value⁶⁾	Analysis value⁷⁾	Recovery (%)	RSD (%)
Pantothenic acid	0.001	0.003	4.831±0.0028	4.832±0.096	150±12	150.75±0.95	100.5	0.63

LOD, limit of detection; LOD=3×signal/noise.
LOQ, limit of quantification; LOQ=10×signal/noise.
Repeatability refers to the results of 5 independent determinations carried out for the same sample on the same day.
Reproducibility refers to the results of 5 independent determinations carried out on a sample by analyzing 3 replicates of the sample at each day for 5 days.
Relative standard deviation (%).
Reference value is the true value provided by NIST.
Analysis value is the experimental value obtained by HPLC assay for pantothenic acid.