| Parameters | Conditions |
|---|---|
| Instrument | Shimadzu LC-20A |
| Injection volume | 10 μL |
| Column | TSK gel ODS-100V (4.6 mm ID × 250 mm, 5 μm) |
| Mobile phase | A: 8 mM perchloric acid (pH 2.10) |
| B: 0.2 mM BTB + 15 mM Na2HPO4 + 7 mM NaOH (pH 11.55) | |
| Flow rate | 1 mL/min |
| Detector | UV 440 nm |
| Oven | 40°C |