Table 6. Method validation of phospholipid analyses with HPLC-ELSD

PLs	Linearity			Sensitivity
	Calibration range (μg/mL)	Calibration curve1)	Coefficient regression (R2)	LOD2) (μg/mL of solvent)	LOQ3) (μg/mL of solvent)
PE	1.95-500.00	y = 13.177x2 + 11,644x − 28,871	0.9999	0.26	0.86
PC	1.95-500.00	y = 16.22x2 + 8,064x − 25,149	0.9999	0.16	0.44
SM	1.95-500.00	y = 11.008x2 + 9,011x − 27,295	0.9997	0.17	0.55
LPE	3.91-500.00	y = 6.0756x2 + 5,311.4x − 10,793	0.9993	0.26	0.81
LPC	1.95-500.00	y = 13.15x2 + 11,156x − 56,065	0.9995	0.14	0.40

y=ax²+bx+c, y and x indicate peak area (mV) and concentration (μg/mL), respectively.
LOD, limit of detection.
LOQ, limit of quantification.