Table 5. Method validation of cholesterol analyses with GC-FID

Cholesterol
Linearity	Calibration range	0.3125-200.00 μg/mL
	Calibration curve1)	y = 10.2x − 0.0061
	Coefficient regression (R2)	0.9997
Sensitivity	LOD2)	0.26 μg/mL of solvent (2.73 mg/100 g of tenderloin)
	LOQ3)	1.17 μg/mL of solvent (3.82 mg/100 g of tenderloin)
		Recovery rate (%)	RSD (%)
Accuracy	Duhangjeongsal
	+0.8 μg/mL	98.02±2.19	2.24
	+0.4 μg/mL	98.53±3.15	3.19
	+0.2 μg/mL	102.10±3.02	2.96
	+0.1 μg/mL	103.03±1.80	1.75

y=ax+b, y and x indicate peak area (mV) and concentration (μg/mL), respectively.
LOD, limit of detection.
LOQ, limit of quantification.
RSD, relative standard deviation.