Table 9. Spike test recovery of different extraction methods for biotin
Sample
Extraction methods
Spike biotin content (μg/g)
Observed biotin content (μg/g)
1)
Recovery (%)
RSD (%)
In house quality control
Autoclave
Non-treated
0.1
0.096±0.001
2)
95.322
0.873
Pepsin-assisted
0.096±0.000
96.212
0.025
Observed biotin content is the value obtained by subtracting the biotin content of the in house quality control.
All values are mean±SD (n=3).