Table 6. Accuracy of different extraction methods for biotin

Sample	Extraction methods		Biotin (μg/100 g)¹⁾		Recovery (%)
Sample	Extraction methods		Reference value²⁾	Analysis value³⁾	Recovery (%)
SRM 1849a (Infant/adult nutritional formula)	Autoclave	Non-treated	199±13	199.735±6.313	100.369
SRM 1849a (Infant/adult nutritional formula)	Autoclave	Pepsin-assisted		196.516±4.762	98.752

All values are mean±SD (n=3).
Reference value is the true value provided by NIST.
Analysis value is the experimental value obtained by immunoaffinity-HPLC/UVD assay for biotin.