Table 2. RT-qPCR conditions for melanogenesis-related genes

Gene	Real-time qPCR condition
MC1R1)	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s, followed by each 95°C, 60°C, 95°C for 15 s
TGF-β12)	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s, followed by each 95°C, 60°C, 95°C for 15 s
Myo5a	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s, followed by each 95°C, 60°C, 95°C for 15 s
Rab27a	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s, followed by each 95°C, 60°C, 95°C for 15 s
β-Actin	95°C for 5 min, followed by undergoing 40 cycles of 95°C for 10 s, 60°C for 20 s, followed by each 95°C, 60°C, 95°C for 15 s

MC1R, melanocortin 1 receptor.
TGF-β1, transforming growth factor-β.