Table 4. Change in the free amino acid of texturized vegetable protein at 0°C for 9 days (unit: μg/mL)

Amino acid	Storage period at 0°C
Amino acid	0 days	1 days	3 days	6 days	9 days
P-Ser	41.65±0.16^NS5)	42.09±0.30^NS	41.70±3.00^NS	43.64±0.57^NS	42.02±0.96^NS
Tau	ND⁴⁾	ND	ND	ND	ND
PEA	3.77±2.42^ab1)	3.94±2.01^ab	6.02±1.58^ab	6.63±0.27^a	1.98±0.31^b
Urea	ND	ND	ND	ND	ND
Asp	ND	ND	ND	ND	ND
Thr	ND	ND	ND	ND	ND
Ser	13.36±0.20^ab	14.17±0.69^ab	14.97±0.52^ab	20.85±0.21^a	8.24±7.98^b
Glu	231.69±0.16^c	252.84±7.24^b	266.12±6.76^ab	270.30±5.20^a	265.72±6.19^ab
Sar	163.26±3.85^b	180.49±7.57^a	189.83±8.56^a	192.60±6.56^a	186.01±5.81^a
Gly	31.27±1.48^b	32.67±2.05^ab	34.13±0.16^ab	33.84±0.25^ab	34.67±0.72^a
Ala	83.42±3.00^b	88.81±6.12^ab	92.69±0.72^a	94.07±3.10^a	94.81±1.65^a
Cit	128.44±14.91^NS	141.59±19.50^NS	146.63±10.59^NS	152.74±5.46^NS	156.82±3.59^NS
a-ABA	3.73±5.28^NS	4.69±6.63^NS	4.38±6.19^NS	6.42±2.18^NS	8.66±0.79^NS
Val	12.94±0.40^NS	11.99±1.43^NS	12.60±0.23^NS	12.64±0.68^NS	12.52±1.05^NS
Cys	325.77±2.98^b	338.90±20.99^ab	363.49±13.79^a	364.08±8.71^a	362.14±11.82^a
Met	15.85±2.73^NS	12.40±5.03^NS	17.20±0.37^NS	17.13±0.41^NS	17.63±3.13^NS
Cysthi	1.04±1.47^NS	ND^b	ND^NS	ND^NS	1.22±1.73^NS
Ile	21.53±0.47^c	22.24±1.05^bc	24.20±1.05^ab	24.44±0.48^a	22.84±0.82^abc
Leu	22.88±0.37^NS	22.58±2.91^NS	25.72±1.13^NS	26.02±0.59^NS	24.28±0.59^NS
Tyr	27.15±0.24^ab	23.31±3.95^b	29.51±1.51^a	30.99±0.98^a	28.10±1.47^ab
Phe	64.73±0.86^b	66.41±2.22^bc	72.42±2.21^a	74.14±2.35^a	71.39±1.82^ab
b-Ala	14.84±2.52^NS	13.03±0.35^NS	16.20±2.86^NS	13.37±0.55^NS	13.60±0.40^NS
b-AiBA	6.89±0.13^b	7.45±0.13^ab	8.50±0.06^a	8.31±0.69^a	8.18±0.51^a
g-ABA	29.70±0.20^b	31.88±0.96^ab	34.06±1.64^a	33.94±0.57^a	33.98±1.10^a
EOHNH2	89.84±0.00^b	96.22±2.77^a	98.35±3.10^a	99.66±1.56^a	99.03±2.33^a
NH3	123.26±0.76^b	117.59±3.46^b	132.44±4.95^a	134.65±2.73^a	133.47±4.06^a
Hylys	ND	ND	ND	ND	ND
Orn	2.75±0.07^c	2.86±0.11^c	3.68±0.03^b	3.01±0.30^c	5.35±0.18^a
Lys	35.73±0.16^c	38.24±0.40^b	39.83±1.20^ab	40.71±0.83^a	39.13±1.17^ab
1Mehis	ND	ND	ND	ND	ND
His	24.36±0.17^c	24.66±0.51^bc	26.86±1.02^ab	26.90±0.65^ab	27.37±1.43^a
3Mehis	ND	ND	ND	ND	ND
Ans	ND	ND	ND	ND	ND
Car	ND	ND	ND	ND	ND
Arg	582.24±3.25^b	616.55±17.49^ab	647.79±21.88^a	651.70±10.24^a	640.75±12.91^a
Hypro	ND^NS	1.59±2.25^NS	0.45±0.64^NS	0.73±1.03^NS	ND^NS
Pro	ND^NS	8.63±8.87^NS	12.18±8.88^NS	1.14±0.79^NS	3.41±4.82^NS
TAA²⁾	2,102.09±31.44	2,217.82±122.98	2,361.95±47.56	2,384.65±56.58	2,343.32±61.46
EAA³⁾	744.53±6.58	776.83±30.65	826.79±27.87	832.97±14.04	816.78±21.75
EAA/TAA (%)	0.35±0.00	0.35±0.01	0.35±0.00	0.35±0.00	0.35±0.00

Values are mean±SD (n=3).
Total free amino acid.
Total essential amino acid (Thr+Val+Met+Ile+Leu+Phe+His+Lys).
Not detected.
Not significant.