Table 3. Change in the free amino acid of texturized vegetable protein at -20°C for 9 days (unit: μg/mL)

Amino acid	Storage period at −20°C
Amino acid	0 days	1 days	3 days	6 days	9 days
P-Ser	41.65±0.16^ab1)	39.38±1.56^b	44.09±2.31^a	42.05±0.10^ab	41.37±1.00^b
Tau	ND⁴⁾	ND	ND	ND	ND
PEA	3.77±2.42^NS5)	2.31±3.27^NS	5.08±0.28^NS	6.22±0.31^NS	4.15±3.18^NS
Urea	ND^NS	ND^NS	ND^NS	ND^NS	12.87±18.20^NS
Asp	ND	ND	ND	ND	ND
Thr	ND	ND	ND	ND	ND
Ser	13.36±0.20^b	13.45±0.41^b	15.43±0.33^a	15.06±0.31^a	14.62±0.85^ab
Glu	231.69±0.16^b	237.58±11.12^b	270.78±15.10^a	266.65±6.66^a	261.13±3.18^a
Sar	163.26±3.85^c	167.61±1.80^bc	190.01±8.36^a	188.93±7.76^a	182.11±6.63^ab
Gly	31.27±1.48^b	30.70±1.41^b	35.84±1.56^a	34.99±2.56^ab	32.66±0.37^ab
Ala	83.42±3.00^b	81.57±3.52^b	97.04±4.58^a	91.83±5.56^ab	91.12±0.71^ab
Cit	128.44±14.91^b	119.13±4.34^b	157.02±5.63^a	144.81±16.53^ab	132.17±1.09^ab
a-ABA	3.73±5.28^NS	ND^NS	8.63±0.41^NS	3.98±5.63^NS	ND^NS
Val	12.94±0.40^ab	12.53±0.58^b	13.92±0.79^a	12.99±0.13^ab	12.96±0.06^ab
Cys	325.77±2.98^b	321.95±15.46^b	369.14±18.53^a	362.25±7.00^a	356.67±3.75^a
Met	15.85±2.73^NS	14.44±0.57^NS	19.44±3.82^NS	18.93±2.14^NS	15.82±0.42^NS
Cysthi	1.04±1.47^NS	ND^NS	1.46±2.06^NS	1.17±1.65^NS	ND^NS
Ile	21.53±0.47^c	21.55±0.89^c	24.67±1.03^a	24.11±0.41^ab	22.89±0.07^bc
Leu	22.88±0.37^c	23.06±1.07^c	26.34±1.07^a	25.53±0.35^ab	24.29±0.04^bc
Tyr	27.15±0.24^cd	26.43±1.82^d	31.67±0.69^a	29.74±0.31^ab	29.10±0.00^bc
Phe	64.73±0.86^b	65.25±2.84^b	75.84±3.05^a	73.13±1.46^a	72.61±0.78^a
b-Ala	14.84±2.52^NS	16.31±0.69^NS	16.60±3.65^NS	15.79±3.66^NS	13.63±0.18^NS
b-AiBA	6.89±0.13^b	6.73±0.35^b	8.31±0.58^a	8.10±0.08^a	8.11±0.10^a
g-ABA	29.70±0.20^b	30.18±1.22^a	34.69±1.57^a	33.71±0.78^a	33.69±0.30^a
EOHNH2	89.84±0.00^b	90.74±3.22^b	98.90±4.41^a	98.39±2.08^a	96.62±1.02^ab
NH3	123.26±0.76^bc	117.83±4.17^c	137.51±6.12^a	130.81±3.61^ab	137.49±1.46^a
Hylys	ND	ND	ND	ND	ND
Orn	2.75±0.07^c	2.34±0.08^c	3.68±0.59^b	3.01±0.01^bc	4.67±0.07^a
Lys	35.73±0.16^b	35.74±1.58^b	41.30±1.95^a	40.24±0.93^a	38.90±0.37^ab
1Mehis	ND	ND	ND	ND	ND
His	24.36±0.17^b	24.26±1.10^b	27.70±1.87^a	27.41±0.18^a	26.75±0.75^ab
3Mehis	ND	ND	ND	ND	ND
Ans	ND	ND	ND	ND	ND
Car	ND	ND	ND	ND	ND
Arg	582.24±3.25^b	580.91±22.98^b	653.22±24.89^a	647.03±13.65^a	637.91±13.45^a
Hypro	ND	ND	ND	ND	ND
Pro	ND^b	14.47±0.55^a	15.30±0.62^a	9.82±11.00^ab	17.45±0.27^a
TAA²⁾	2,102.09±31.44	2,096.45±79.92	2,423.61±114.62	2,356.68±86.44	2,321.76±57.53
EAA³⁾	744.53±6.58	742.00±30.04	841.13±36.53	829.13±14.04	813.23±15.49
EAA/TAA (%)	0.35±0.00	0.35±0.00	0.35±0.00	0.35±0.01	0.35±0.00

Values are mean±SD (n=3).
Total free amino acid.
Total essential amino acid (Thr+Val+Met+Ile+Leu+Phe+His+Lys).
Not detected.
Not significant.