Table 1. Experimental design of fermentation conditions for black soybean
| Fermentation conditions | Coded level1) |
| −2 | −1 | 0 | 1 | 2 |
| X1 | Enzyme concentration (%) | 0.1 | 0.2 | 0.3 | 0.4 | 0.5 |
| X2 | Fermentation time (min) | 20 | 40 | 60 | 80 | 100 |
The experiment was designed based of five-level central composite design.