Table 5. Threshold cycle (Ct) values of sensitivity in ultra-fast quantitative real-time PCR for Oncorhynchus keta and Oncorhynchus mykiss

Species	Concentration (ng/μL)	Ct value			Mean	SD1)
		Repeat 1	Repeat 2	Repeat 3
Oncorhynchus keta	10	19.28	19.16	19.17	19.20	0.05
	5	20.13	20.16	20.27	20.19	0.06
	2.5	21.11	21.17	21.26	21.18	0.06
	1	22.69	23.19	23.29	23.06	0.26
	0.1	24.83	-	-	24.83	-
	Negative	-2)	-	-	-	-
Oncorhynchus mykiss	10	15.67	16.17	16.17	16.00	0.24
	5	16.72	16.20	16.84	16.59	0.28
	2.5	17.58	17.20	16.97	17.25	0.25
	1	19.11	19.13	18.97	19.07	0.07
	0.1	22.15	22.86	22.83	22.61	0.33
	Negative	-3)	-	-	-	-

SD, standard deviation.
-, Ct value of 24 or more.
-, Ct value of 20 or more.