Table 1. Primer sequence designed in this study

Species	Sequences	Product size (bp)	Tm (°C)	Target gene
Oncorhynchus keta	Forward	5’-TCT AGG GGA TGA CCA GAT CTA CA -3’	184	59.3	COI1)
	Reverse	5’-GGA CGG AGG TAG GAG TCA GA -3’		59.7
Oncorhynchus mykiss	Forward	5’-CCC TAA TAA TCG GAG CCC CT -3’	133	58.3	COI
	Reverse	5’-ACT GTT CAT CCA GTA CCC GC -3’		59.7

COI, cytochrome C oxidase subunit I.