Table 2. A gradient condition of HPLC mobile phases for β-carotene analysis

Time (min)	Mobil phase A1) (%)	Mobil phase B2) (%)
0	75	25
8	75	25
50	10	90
55	10	90
57	75	25
65	75	25

Methanol:buthanol:water=89.5:10.0:0.5 (v/v/v).
Methanol:buthanol:water=60:10:30 (v/v/v).