| Analysis condition | UPLC-FLD | HPLC-FLD |
|---|---|---|
| Instrument | WATERS ACQUITY | HITACHI Chromaster |
| Column | WATERS Xselect CSH C18 | HITACHI LaChrom C18-AQ |
| Mobile phase (ACN : MeOH : Water) | 15 : 20 : 65 (v/v/v) | 10 : 30 : 60 (v/v/v) |
| Flow rate | 0.2 mL/min | 1.0 mL/min |
| Run time | 10 min | 20 min |
| Column temperature | 40°C | 40°C |
| Injection volume | 10 | 10 |
| FL detector wavelength | EX 360 nm, Em 440 nm | EX 365 nm, Em 450 nm |