Table 2. Detection of E. coli, L. monocytogenes, Salmonella spp., and S. aureus in samples collected from each production process of button mushroom in farm A, B, C and D. Salmonella spp. was detected in the deep-stacking medium (Farm C, D), 1st and 2nd turned compost (Farm D). Salmonella spp. was negative in the other samples. S. aureus was negative in all samples

Production stages Farm A Farm B Farm C Farm D
Sample type E. coli L. monocytogenes E. coli L. monocytogenes E. coli L. monocytogenes E. coli L. monocytogenes
Raw materials
 Rice straw +1)(3/3) +(2/3) -2) - -2) - ND ND
 Chicken manure ND ND +(1/3) ND +1)(3/3) ND ND ND
 Chicken manure (undried) - - - - - - ND ND
 Wheat straw +(3/3) +(2/3) - - - - +(2/3) ND
 Heaped wheat straw - - - - +(3/3) +(2/3) - -
 Heaped rice straw - - ND ND - - - -
 Liquid fertilizer - - - - +(3/3) +(2/3) - -
Composting
 Deep-stacking medium +(3/3) +(2/3) +(3/3) ND +(2/3) +(2/3) +(3/3) ND
 1st turned compost +(3/3) +(2/3) +(3/3) ND ND ND +(3/3) ND
 2nd turned compost ND +(2/3) ND ND ND ND ND ND
 3rd turned compost +(3/3) ND ND ND ND ND +(1/3) ND
 4th turned compost +(3/3) ND +(3/3) ND ND ND ND ND
Pasteurization
 Pasteurized compost ND ND ND ND ND ND ND ND
Fruiting
 Spawn - - ND ND ND ND ND ND
 Colonized bed ND ND ND ND ND ND ND ND
 Casing soil ND ND ND ND ND ND +(2/3) ND
Harvesting
 Casing soil layer +(2/3) ND ND ND +(1/3) ND +(1/3) ND
 Harvested mushroom +(1/3) ND ND ND +(2/3) ND ND ND
 Knife ND ND ND ND ND ND ND ND
 Basket ND ND - - ND ND ND ND
 Glove +(2/3) ND ND ND +(3/3) ND +(1/3) ND
Packaging
 Bench ND ND ND ND ND ND ND ND
 Scale - - ND ND - ND ND ND
 Packaged mushroom ND ND ND ND ND ND ND
+, positive detection.
-, no sample.
ND, not detected sample.