Table 2. Box-Behnken design of independent variables with response variables

Run	Independent variables			Measured responses
Run	X₁: Soaking time (h)	X₂: Germination temperature (°C)	X₃: Germination day	TPC (mg GE/g)	TFC (mg CE/g)	DPPH RSA (μM TE/g)	Soluble protein (mg/g)
1	6 (−1)¹⁾	25 (0)	6 (+1)	1.25±0.05^f²⁾	0.50±0.01^b	3.43±0.06^b	76.7±2.58^a
2	18 (+1)	20 (−1)	4 (0)	1.06±0.03^h	0.40±0.04^e	2.49±0.07^e	60.9±2.92^bcdef
3	6 (−1)	20 (−1)	4 (0)	1.43±0.01^c	0.33±0.01^fg	2.42±0.07^ef	60.2±2.45^bcdef
4	12 (0)	30 (+1)	2 (−1)	1.01±0.01ⁱ	0.26±0.01^hi	2.02±0.06^h	49.1±1.24^g
5	12 (0)	25 (0)	4 (0)	1.27±0.005^f	0.42±0.01^de	2.51±0.08^e	66.8±6.38^abcd
6	12 (0)	25 (0)	4 (0)	1.26±0.005^f	0.34±0.01^f	2.26±0.09^fg	63.5±1.50^bcde
7	18 (+1)	30 (+1)	4 (0)	1.53±0.005^b	0.29±0.01^gh	3.18±0.07^c	69.3±2.59^abc
8	18 (+1)	25 (0)	6 (+1)	1.53±0.03^b	0.54±0.04^a	3.65±0.10^a	65.9±5.52^bcde
9	12 (0)	30 (+1)	6 (+1)	2.10±0.00^a	0.33±0.004^fg	2.97±0.02^c	62.9±2.05^bcde
10	12 (0)	25 (0)	4 (0)	1.34±0.005^d	0.45±0.02^cd	2.75±0.03^d	66.4±1.20^abcd
11	12 (0)	20 (−1)	6 (+1)	1.21±0.02^g	0.32±0.01^fg	2.59±0.12^de	51.2±1.38^fg
12	6 (−1)	30 (+1)	4 (0)	1.29±0.02^ef	0.30±0.01^gh	2.59±0.12^de	70.2±0.00^ab
13	6 (−1)	25 (0)	2 (−1)	0.91±0.02^j	0.23±0.01ⁱ	2.13±0.04^gh	56.8±1.49^defg
14	18 (+1)	25 (0)	2 (−1)	0.94±0.03^j	0.26±0.04^hi	1.71±0.08ⁱ	55.4±9.55^efg
15	12 (0)	20 (−1)	2 (−1)	0.94±0.01^j	0.32±0.01^fg	2.14±0.08^gh	70.7±1.09^ab
16	12 (0)	25 (0)	4 (0)	1.44±0.005^c	0.32±0.00^fg	2.55±0.16^de	55.5±11.56^efg
17	12 (0)	25 (0)	4 (0)	1.31±0.01^de	0.46±0.02^c	2.50±0.25^e	58.9±4.29^cdefg

Values were coded levels for process variables according to BBD. TPC, total phenolic content (mg GE/g); TFC, total flavonoid content (mg CE/g); DPPH RSA, DPPH radical scavenging activity (μM TE/g); GE, gallic acid equivalent; CE, catechin equivalent; TE, trolox equivalent.
Results represented by mean±SD (n=3).