OT | SC | LA | CM | FE | ||
---|---|---|---|---|---|---|
Alanine | Before | 351.91±2.67 | 236.23±0.76 | 256.53±2.28 | 304.72±5.52 | 227.29±6.55 |
After | 344.19±5.76 | 298.08±7.96 | 287.55±4.38 | 353.37±1.21 | 276.63±9.45 | |
t-value | 3.53 | −10.04 | −6.59 | −10.22 | −24.07 | |
Arginine | Before | 28.91±0.30 | 28.94±0.35 | 26.48±0.23 | 20.80±0.29 | 3.97±0.20 |
After | 51.81±0.44 | 66.96±1.05 | 58.43±1.52 | 49.40±1.96 | 40.62±2.31 | |
t-value | −228.71 | −38.20 | −25.76 | −24.17 | −24.07 | |
Aspartic acid | Before | 117.93±0.11 | 103.13±0.23 | 108.00±1.01 | 119.71±0.28 | 60.45±1.48 |
After | 117.10±1.94 | 129.08±3.24 | 123.61±1.97 | 141.69±0.44 | 82.72±0.48 | |
t-value | 0.63 | −12.23 | −7.39 | −43.21 | −16.07 | |
Cysteine | Before | 2.81±1.85 | 34.63±0.42 | 72.22±0.75 | 49.44±2.04 | 61.97±1.18 |
After | 58.50±1.24 | 76.35±2.79 | 64.34±1.39 | 68.78±0.41 | 59.30±2.22 | |
t-value | −25.50 | −24.92 | 5.22 | −11.19 | 3.65 | |
Glutamic acid | Before | 206.26±2.55 | 198.98±3.16 | 205.40±2.61 | 214.69±0.63 | 116.95±2.91 |
After | 208.02±4.93 | 295.11±27.68 | 252.95±4.99 | 279.23±1.33 | 178.27±31.54 | |
t-value | −0.33 | −5.54 | −8.85 | −46.49 | −2.52 | |
Glycine | Before | 153.54±1.21 | 116.88±0.88 | 128.05±0.95 | 144.67±3.52 | 92.70±2.38 |
After | 147.14±2.71 | 146.65±3.03 | 142.55±2.01 | 165.21±0.59 | 114.82±1.92 | |
t-value | 2.31 | −10.77 | −6.94 | −7.07 | −69.03 | |
Histidine | Before | 64.50±0.51 | 57.75±0.28 | 57.05±0.95 | 63.78±0.08 | 40.73±0.62 |
After | 69.29±2.07 | 83.53±4.56 | 75.51±2.00 | 83.54±0.02 | 65.33±0.13 | |
t-value | −4.35 | −8.51 | −8.83 | −290.84 | −46.41 | |
Isoleucine | Before | 125.29±0.07 | 81.74±0.26 | 101.31±1.22 | 106.65±8.40 | 63.72±0.62 |
After | 115.67±1.54 | 129.43±3.88 | 108.75±2.03 | 117.82±0.52 | 88.96±7.30 | |
t-value | 9.23 | −18.63 | −3.24 | −1.77 | −4.51 | |
Leucine | Before | 248.90±1.46 | 181.45±0.60 | 205.52±2.23 | 235.02±8.98 | 115.62±2.86 |
After | 237.35±3.85 | 253.67±7.22 | 228.72±4.67 | 267.78±0.61 | 173.19±5.67 | |
t-value | 6.82 | −15.44 | −4.76 | −4.83 | −29.07 | |
Lysine | Before | 184.12±2.35 | 182.84±3.89 | 183.22±2.11 | 168.02±0.42 | 117.35±2.89 |
After | 183.88±2.77 | 227.14±6.21 | 216.18±5.32 | 204.58±0.53 | 152.58±6.83 | |
t-value | 0.79 | −27.00 | −6.28 | −53.95 | −5.13 | |
Methionine | Before | 69.64±0.92 | 43.27±0.04 | 64.54±0.56 | 82.69±9.45 | 42.39±0.69 |
After | 60.42±1.36 | 74.45±2.11 | 57.12±0.94 | 83.14±0.25 | 64.80±5.09 | |
t-value | 29.41 | −21.28 | 6.99 | −0.07 | −7.20 | |
Phenyl alanine | Before | 196.10±0.47 | 134.96±0.64 | 168.03±1.60 | 179.75±23.40 | 99.07±1.99 |
After | 183.63±2.16 | 210.35±7.42 | 166.09±3.17 | 193.08±0.44 | 152.62±14.84 | |
t-value | 10.43 | −15.73 | 0.58 | −0.79 | −5.90 | |
Proline | Before | 222.90±0.85 | 178.59±1.00 | 182.46±0.96 | 263.46±2.53 | 171.65±9.88 |
After | 216.60±4.52 | 215.67±3.12 | 204.98±7.30 | 303.99±0.34 | 206.17±8.94 | |
t-value | 2.43 | −12.72 | −3.86 | −19.94 | −52.19 | |
Serine | Before | 118.80±0.16 | 107.67±0.81 | 116.06±0.81 | 132.19±0.03 | 76.42±2.52 |
After | 117.56±2.99 | 141.11±1.89 | 134.76±2.23 | 155.65±1.40 | 110.37±0.14 | |
t-value | 0.56 | −43.47 | −8.69 | −23.24 | −20.21 | |
Threonine | Before | 75.94±0.07 | 68.51±1.10 | 70.65±0.53 | 81.26±0.55 | 48.31±1.20 |
After | 78.30±2.23 | 95.96±1.22 | 90.59±1.29 | 99.92±0.86 | 74.18±1.51 | |
t-value | −1.55 | −340.51 | −15.53 | −84.27 | −118.43 | |
Tyrosine | Before | 206.86±0.78 | 139.60±1.17 | 168.92±2.30 | 197.12±20.70 | 115.52±2.23 |
After | 195.19±1.90 | 219.24±7.21 | 173.54±3.58 | 214.57±0.63 | 161.84±15.01 | |
t-value | 14.67 | −18.65 | −1.11 | −1.16 | −5.13 | |
Valine | Before | 221.39±2.45 | 120.04±0.15 | 134.11±1.06 | 158.17±4.34 | 86.62±2.27 |
After | 158.35±3.15 | 177.35±5.26 | 154.28±2.78 | 182.18±0.68 | 137.51±1.30 | |
t-value | 128.11 | −15.85 | −7.43 | 0.93 | −74.47 | |
γ-Amino butyric acid | Before | 40.13±0.67 | 39.24±0.07 | 46.85±0.52 | 29.77±4.24 | 35.71±0.91 |
After | 34.79±0.03 | 49.29±4.49 | 40.64±0.71 | 26.91±0.10 | 39.96±4.43 | |
t-value | 10.72 | −3.12 | 7.10 | 0.93 | −1.13 | |
Total free nitrogenous content | Before | 2,635.93±6.56 | 2,054.42±10.40 | 2,295.37±22.66 | 2,551.87±90.23 | 1,576.41±42.14 |
After | 2,577.79±45.59 | 2,889.41±98.25 | 2,580.60±52.28 | 2,990.84±7.58 | 2,179.88±27.65 | |
t-value | 2.11 | −13.44 | −5.38 | −6.35 | −58.90 |