Table 2. HPLC gradient conditions for retinol and β-carotene simultaneous analyses
Time (min) | A1) (%) | B2) (%) |
0 | 75 | 25 |
8 | 75 | 25 |
50 | 10 | 90 |
55 | 10 | 90 |
57 | 75 | 25 |
65 | 75 | 25 |
Mobile phase A: methanol:butanol:water=89.5:10:0.5 (v/v/v).
Mobile phase B: methanol:butanol:water=60:10:30 (v/v/v).