Table 1. Analytical conditions of HPLC for analysis of astragalin

Detector	UV detector (BAP ACQUITY UPLC TUV detector)
Wavelength	254 nm
Column	ACQUITY UPLC BEH C18 (2.1×100 mm, 1.7 μm)
Gradient	A: 0.1% formic acid in deionized waterB: acetonitrile
	Time (min)	A (%)	B (%)
	Initial	95.0	5.0
	5	95.0	5.0
	35	0.0	100.0
	45	0.0	100.0
	50	95.0	5.0
	55	95.0	5.0
Flow rate	0.1 mL/min
Injection volume	1.0 μL
Column temp	40°C