Detector | UV detector (BAP ACQUITY UPLC TUV detector) | ||
Wavelength | 254 nm | ||
Column | ACQUITY UPLC BEH C18 (2.1×100 mm, 1.7 μm) | ||
Gradient | A: 0.1% formic acid in deionized water | ||
Time (min) | A (%) | B (%) | |
Initial | 95.0 | 5.0 | |
5 | 95.0 | 5.0 | |
35 | 0.0 | 100.0 | |
45 | 0.0 | 100.0 | |
50 | 95.0 | 5.0 | |
55 | 95.0 | 5.0 | |
Flow rate | 0.1 mL/min | ||
Injection volume | 1.0 μL | ||
Column temp | 40°C |