Table 1. Analytical conditions of HPLC for analysis of astragalin

Detector UV detector (BAP ACQUITY UPLC TUV detector)
Wavelength 254 nm
Column ACQUITY UPLC BEH C18 (2.1×100 mm, 1.7 μm)
Gradient A: 0.1% formic acid in deionized waterB: acetonitrile
Time (min) A (%) B (%)
Initial 95.0 5.0
5 95.0 5.0
35 0.0 100.0
45 0.0 100.0
50 95.0 5.0
55 95.0 5.0
Flow rate 0.1 mL/min
Injection volume 1.0 μL
Column temp 40°C