Table 2. HPLC gradient conditions of mobile phases for isorhamnetin, naringin and hesperidin analyses

Isorhamnetin	Time (min)	Mobile phase A (%)1)	Mobile phase B (%)2)
	0	100	0
	5	90	10
	35	60	40
	50	100	0
	55	100	0
	65	100	0
Naringin & hesperidin	Time (min)	Mobile phase A (%)3)	Mobile phase B (%)4)
	0	85	15
	35	35	65
	45	0	100
	50	0	100
	55	85	15

Water:acetic acid:acetonitrile=94.5:0.5:5(v/v/v).
Acetonitrile.
Water (1% acetic acid).
Acetonitrile (1% acetic acid).