Table 1. Characteristics and activity of various cell-wall degrading enzymes used in the study

Enzyme Characteristics Experimental activity (unit/mL)1)
Sources Optimal pH Optimal temperature (℃) Enzyme activity Cellulase Hemicellulase
AMG 300 L Aspergillus niger 4.5 60 Glucoamylase 121.2±7.62) 129.1±2.8
Celluclast 1.5 L Trichoderma reesei 4.5-6.0 50-60 Cellulase 200.5±5.5 645.3±27.3
Pectinex Ultra SP-L Aspergillus aculeatus Polygalacturonase 139.7±13.3 67.1±5.8
Shearzyme pluse Trichoderma reesei 4.5-6.0 50-60 Cellulase 136.2±13.6 508.4±16.3
Viscozyme L Aspergillus aculeatus 3.5-5.5 25-55 Arabanase, cellulase β-glucanase, hemicellulase, xylanase 376.7±6.1 255.8±25.8
One unit of cellulase or hemicellulase activity was expressed as the number of μmoles of glucose or xylose produced for 1 min at pH 5.0 and 50℃, respectively.
Mean±SD (n=3).