Table 2. Elution profile for the analysis of free amino acids

Time	A1)	B	C
	100	0	0
0.5	99.5	0.5	0
10.0	96.5	3.5	0
15.0	96	4	0
18.0	96	4	0
19.0	91	9	0
29.5	83	17	0
40.0	0	50	50
450	100	0	0
55.0	100	0	0

A, Waters AccQ Taq Eluent A: DW = 1:10 (90:900); B, acetonitrile (ACN); C, pure water.