Table 1. The operating condition of UPLC for analysis of astaxanthin

	Condition
Column	YMC carotenoid, 4.6 mm × 250 mm
Injection volume	10 μL
Column oven temperature	25°C
Flow rate	0.5 mL/min
Detector	PDA detector, 474 nm
Mobile phase	A : methanol
	B : methyl tert-butyl ether
	C : 1% phosphoric acid
	Min	A(%)	B(%)	C(%)
	0	81	15	4
	15	66	30	4
	23	16	80	4
	27	16	80	4
	27.1	81	15	4
	35	81	15	4