Table 1. The contents of hesperidin and hesperetin of the hydrolyzed hesperidin by three commercial enzymes
(unit: ppm)
Enzyme | Hydrolyzed hesperidin |
pH 4.0 | pH 5.0 | pH 6.0 |
2 h | 24 h | 2 h | 24 h | 2 h | 24 h |
Hesperidin | Hesperetin | Hesperidin | Hesperetin | Hesperidin | Hesperetin | Hesperidin | Hesperetin | Hesperidin | Hesperetin | Hesperidin | Hesperetin |
Viscozyme L | 5.82±0.01c1) | 2.78±0.00a | 4.26±0.02c | 3.55±0.02a | 6.06±0.03c | 2.63±0.02a | 3.20±0.03c | 3.83±0.02a | 5.67±0.01c | 2.48±0.00a | 4.07±0.01b | 3.31±0.03b |
AMG 300L | 11.27±0.07a | 0.45±0.00c | 9.10±0.02a | 1.32±0.00c | 8.92±0.02b | 0.59±0.00c | 8.51±0.03a | 1.35±0.00c | 11.19±0.03a | 0.38±0.02c | 9.94±0.02a | 0.91±0.03c |
Pectinex Ultra Pulp | 9.60±0.11b | 1.22±0.00b | 4.49±0.02b | 3.18±0.01b | 10.22±0.07a | 1.41±0.01b | 4.92±0.00b | 3.64±0.01b | 9.21±0.00b | 1.08±0.02b | 3.23±0.00c | 3.67±0.03a |
a-cMeans within the same column with different superscript letters are significant different by Duncan’s multiple range test at p<0.05 (n=3).