Table 4. Inhibitory effects of 70% methanolic extract of D. takeshimana from Ulleung Island and its organic solvent-soluble portions against α-glucosidase
Samples | Inhibition (%) | IC50(μg/mL) |
500 μg/mL | 250 μg/mL | 125 μg/mL | 62.5 μg/mL | 31.3 μg/mL |
70% MeOH ext. | 47.4±0.91)d2) | 38.8±0.9d | 29.3±0.5d | 19.2±0.4d | 7.3±0.4d | >500a |
n-Hexane layer | 99.9±1.6a | 90.8±1.5a | 80.9±1.3a | 63.8±1.0a | 47.7±0.7a | 55.5±0.4d |
EtOAc layer | 64.9±0.9c | 53.4±0.9c | 43.4±0.8b | 29.1±0.7b | 12.1±0.5c | 229.3±2.3b |
n-BuOH layer | 24.0±0.6e | 19.0±0.5e | 11.3±0.3e | 5.8±0.2e | 2.7±0.2e | >500a |
H2O layer | 5.2±0.5f | 2.9±0.3f | 1.5±0.2f | 0.5±0.1f | 0.1±0.03f | >500a |
Acarbose3) | 69.1±1.1b | 56.7±1.1b | 41.2±0.8c | 29.3±0.7c | 15.8±0.5b | 193.1±2.1c |
1)Inhibitory effects are expressed as the mean±SD of triplicate experiments.
2)Different letters (a-f) within the same column indicate significant differences (p<0.05).
3)Acarbose was used as a positive control.