Table 3. ABTS+ radical scavenging activity of 70% methanolic extract of D. takeshimana from Ulleung Island and its organic solvent-soluble portions
Samples | Scavenging activity (%) | IC50(μg/mL) |
500 μg/mL | 250 μg/mL | 125 μg/mL | 62.5 μg/mL | 31.3 μg/mL |
70% MeOH ext. | 80.8±1.31)c2) | 56.0±1.0c | 26.5±0.8c | 14.9±0.6d | 6.8±0.2d | 200.3±2.7d |
n-Hexane layer | 32.0±0.5f | 12.2±0.4f | 5.3±0.4f | 3.1±0.3f | 0.8±0.1f | >500a |
EtOAc layer | 95.1±1.2b | 83.3±1.1b | 74.2±1.1b | 42.9±0.8b | 18.5±0.5b | 72.7±1.2e |
n-BuOH layer | 88.2±1.0d | 64.2±0.9d | 36.9±1.0d | 22.0±0.8c | 10.7±0.4c | 165.2±2.5c |
H2O layer | 64.0±0.9e | 33.4±0.7e | 16.5±0.6e | 5.6±0.2e | 3.1±0.1e | 365.9±3.8b |
(+)-Catechin3) | 99.9±1.8a | 95.8±1.6a | 88.5±1.5a | 65.3±1.0a | 31.9±0.9a | 18.4±0.3f |
1)Radical scavenging activities are expressed as the mean±SD of triplicate experiments.
2)Different letters (a-f) within the same column indicate significant differences (p<0.05).
3)(+)-Catechin was used as a positive control.