Table 2. DPPH radical scavenging activity of 70% methanolic extract of D. takeshimana from Ulleung Island and its organic solvent-soluble portions
Samples | Scavenging activity (%) | IC50(μg/mL) |
500 μg/mL | 250 μg/mL | 125 μg/mL | 62.5 μg/mL | 31.3 μg/mL |
70% MeOH ext. | 77.3±1.31)c2) | 55.9±0.8c | 31.8±0.5c | 14.5±0.6d | 12.5±0.3c | 213.9±3.4d |
n-Hexane layer | 33.8±0.9f | 20.7±0.2f | 14.9±0.4f | 7.2±0.3f | 3.2±0.3f | >500a |
EtOAc layer | 89.5±1.2b | 66.3±1.0b | 48.8±0.6b | 28.9±0.4b | 20.5±0.4b | 127.3±2.1e |
n-BuOH layer | 71.0±1.0d | 50.1±0.6d | 29.4±0.5d | 19.4±0.3c | 12.6±0.2d | 247.3±3.5c |
H2O layer | 51.2±0.8e | 35.0±0.3e | 23.1±0.3e | 12.9±0.2e | 8.7±0.1e | 492.2±4.6b |
(+)-Catechin3) | 99.9±1.8a | 95.6±1.4a | 88.2±1.3a | 73.4±1.3a | 53.5±1.0a | 22.2±0.6f |
1)Radical scavenging activities are expressed as the mean±SD of triplicate experiments.
2)Different letters (a-f) within the same column indicate significant differences (p<0.05).
3)(+)-Catechin was used as a positive control.