Parameters | Condition | ||
---|---|---|---|
HPLC model | Waters 1500-series | ||
Column | Agilent Eclips XDB-C18 (4.6×150 mm, 5 μm) | ||
Detector | UV 254 nm | ||
Flow rate | 0.5 mL/min | ||
Column temperature | 40℃ | ||
Injection volume | 20 μL | ||
Mobile phase | A: 1% formic acid in water, B: Acetonitrile | ||
Gradient | Time (min) | A (%) | B (%) |
0 | 90 | 10 | |
7 | 90 | 10 | |
28 | 45 | 55 | |
32 | 30 | 70 | |
35 | 90 | 10 | |
40 | 90 | 10 |