Table 2. Analytical condition of HPLC for analysis of ellagic acid

Parameters Condition
HPLC model Waters 1500-series
Column Agilent Eclips XDB-C18 (4.6×150 mm, 5 μm)
Detector UV 254 nm
Flow rate 0.5 mL/min
Column temperature 40℃
Injection volume 20 μL
Mobile phase A: 1% formic acid in water, B: Acetonitrile
Gradient Time (min) A (%) B (%)
0 90 10
7 90 10
28 45 55
32 30 70
35 90 10
40 90 10