Table 1. Analytical condition of HPLC for analysis of quercetin

Parameters	Condition
HPLC model	Waters 1500-series
Column	PhenoSphereTM ODS(2) column (4.6×250 mm, 5 μm)
Detector	UV 370 nm
Flow rate	1.0 mL/min
Column temperature	40℃
Injection volume	20 μL
Gradient	Time (min)	% A1)	% B2)
	2	80	20
	25	55	45
	30	0	100
	31	80	20

¹⁾0.1% trifluoroacetic acid in water.
²⁾0.1% trifluoroacetic acid in acetonitrile.