Table 1. HPLC-ELSD analysis conditions of isomaltooligosaccharides manufactured by one-step method with puffed rice flour

Instrument	Agilent Technologies 1260 infinity
Column	shodex HILICpak VN-50 4D
Mobile phase	Gradient conditon
	Time table (min)	Acetonitrile (%)
	0	89
	25	89
	30	81
	40	80
	75	75
	80	73
	80.1	89
	85	89
Flow rate	1.0 mL/min
Temperature	65℃
Injection volume	10 μL
Detector	ELSD
	Temperature	90℃
	Gain	10