Table 2. Operating conditions of amino acid auto-analyzer

Instrument S430 (SYKAM)
Column Cation separation column (LCA K07/Li)
Column size 4.6×150 mm
Column temperature 37-74℃
Flow rate Buffer 0.45 mL/min, reagent 0.25 mL/min
Buffer pH range 2.90-7.95
Wave length 440 nm and 570 nm