Table 2 Compar ison of isoflavone contents in the unfermented and fermented raw and HPSs sprouting

Contents (μg/g)	Raw soybean		Sprouting length (cm)
			0		1		2		4
			UFHPS1)	FHPS	UFSHPS	FSHPS	UFSHPS	FSHPS	UFSHPS	FSHPS	UFSHPS	FSHPS
	β-glycosidases
	Daidzin	340.49±13.622)^c3)	33.41±1.34^e	364.70±15.59^c	107.24±5.36^d	341.82±17.09^c	22.51±1.13^f	410.77±20.54^d	20.57±1.03^f	468.46±23.42^a	24.19±1.21^f
Glycitin	149.68±5.99^b	41.73±1.67^e	151.88±6.08^b	78.81±3.94^d	179.76±8.99^a	28.50±1.43^f	179.05±8.95^a	17.03±0.85^g	122.67±6.13^c	25.27±1.26^f
Genistin	362.43±15.50^d	13.83±0.55^f	397.89±15.92^c	33.76±1.69^e	388.07±19.40^c	2.35±0.12^g	409.08±20.45^b	0.56±0.03^h	471.52±23.58^a	4.49±0.22^f
Total	852.60±34.10^d	88.97±3.56^f	914.47±36.58^c	219.81±10.99^e	909.65±45.48^c	53.36±2.67^g	998.90±49.95^b	38.16±1.91^h	1,062.65±53.13^a	53.95±2.70^g
Malonyl-β-glycosidases
Daidzin	52.89±3.17^a	44.71±2.68^b	15.12±0.91^d	4.15±0.91^e	28.54±0.25^c	15.51±0.78^d	21.99±1.10^c	6.73±0.34^e	23.25±1.16^c	12.09±0.60^d
Glycitin	15.40±0.92^b	14.49±0.87^b	11.03±0.66^b	10.90±0.66^c	16.81±0.65^a	18.63±0.93^a	13.19±0.66^b	9.74±0.49^c	14.78±0.74^b	12.76±0.64^b
Genistin	72.70±4.36^a	64.28±3.86^b	20.09±1.21^f	18.12±1.21^g	37.37±1.09^e	20.08±1.00^f	46.25±2.31^d	29.43±1.47^f	55.86±2.79^c	28.94±1.45^f
Total	140.99±8.46^a	123.48±7.41^b	46.24±7.41^f	33.17±2.77^g	82.72±1.99^d	54.22±2.71^e	81.43±4.07^d	45.90±2.30^f	93.89±4.69^c	53.79±2.69^e
Acetyl-β-glycosidases
Daidzin	tr4)	tr	ND5)	ND	ND	ND	ND	ND	ND	ND
Glycitin	5.36±0.27^a	4.80±0.24^b	6.18±0.31^a	5.60±0.28^a	1.39±0.07^c	1.32±0.05^c	2.47±0.12^c	1.79±0.09^c	1.77±0.08^c	1.90±0.10^c
Genistin	2.68±0.13^b	2.40±0.12^b	3.09±0.15^a	2.80±0.14^b	0.69±0.03^d	0.66±0.03^d	1.23±0.06^c	0.89±0.04^d	0.88±0.04^d	0.95±0.05^d
Total	8.04±0.40^b	7.20±0.36^c	9.27±0.46^a	8.40±0.42^b	2.08±0.10^e	1.98±0.10^e	3.70±0.19^d	2.68±0.13^e	2.65±0.13^e	2.85±0.14^e
Aglycones
Daidzein	19.00±1.14^g	181.83±10.91^c	8.99±0.54^h	138.55±8.31^d	26.05±1.56^f	186.08±11.16^c	59.71±3.58^e	243.78±14.63^b	53.07±3.18^e	277.71±16.66^a
Glycitein	6.58±0.39^e	36.98±2.22^c	38.25±2.30^c	30.05±1.80^c	12.24±0.73^d	46.01±2.76^b	19.37±1.16^d	62.00±3.72^a	16.24±0.97^d	48.34±2.90^b
Genistein	14.80±0.89^d	131.53±7.89^b	6.83±0.41^e	120.55±7.23^c	10.00±0.60^d	129.52±7.77^c	16.04±0.96^d	139.27±8.36^b	11.49±0.69^d	165.20±9.91^a
Total	40.38±2.42^h	350.34±21.02^c	54.07±3.24^g	289.15±17.35^d	48.29±2.90^h	361.61±21.70^c	95.12±5.71^e	445.05±26.70^b	80.80±4.85^f	491.25±29.48^a
Isoflavone totals	1,042.01±62.52^c	569.99±34.20^e	1,024.05±61.44^c	550.03±33.00^e	1,042.74±62.56^c	471.17±28.27^g	1,179.15±70.75^b	531.79±31.91^f	1,239.99±74.40^a	601.84±36.11^d

Abbreviation: UFHSP, unfermented high-protein soybean; FHSP, fermented high-protein soybean; UFSHPS, unfermented steam high-protein soybean; and FSHPS, fermented steam high-protein soybean.
All values are presented as the mean±SD of triplicate determination.
All values within a column with different superscript letters are significantly different from each other at p<0.05 by Duncan’s multiple range test.
tr: trace (< 0.002 μg/g).
ND, not detected.