Table 1
Primer sequences and PCR conditions used to gene analysis

Target	Genes	Primer sequence (5’-3’)	PCR condition
B. cereus toxin	hbl1)A-F	GCTAATGTAGTTTCACCTGTAGCAAC	95°C, 5 min → (95°C, 45 sec → Tm°C, 45 sec → 72°C, 2 min) 35 cycles → 72°C, 5 min
	hblA-R	AATCATGCCACTGCGTGGACATATAA
	hblD-F	AATCAAGAGCTGTCACGAAT
	hblD-R	CACCAATTGACCATGCTAAT
	nhe2)B-F	ACTTATGGCAGTATTTGCAGCAGGA
	nheB-R	TGCAACGCTGTAATGCAGTATCAA
	nheC-F	GACCAGCAGGATTCCCAGATGTAAT
	nheC-R	CCACGCCTTCATGTAATTTTTCTGT
	ces3)-F	GGTGACACATTATCATATAAGGTG
	ces-R	GTAAGCGAACCTGTCTGTAACAACA
Bacteriocin	bmy4)A-F	GAAGGACACGGCAGAGAGTC
	bmyA-R	CGCTGATGACTGTTCATGCT
	itu5)A-F	GATGCGATCTCCTTGGATGT
	ituA-R	ATCGTCATGTGCTGCTTGAG
	suf6)A-F	ACAGTATGGAGGCATGGTC
	sufA-R	TTCCGCCACTTTTTCAGTTT
Biogenic amine	hdc7)-F	GATGGTATTTCKTATGA
	hdc-R	CAAACACCAGCATCTTC
	tdc8)-F	CAAACACCATCTTC
	tdc-R	ACATAGTCAACCATRTTGAA

hbl, hemolytic enterotoxin gene.
nhe, non-hemolytic enterotoxin gene.
ces, hemolytic enterotoxin gene.
bmyA, hemolytic enterotoxin gene.
ituA, hemolytic enterotoxin gene.
sufA, hemolytic enterotoxin gene.
hdc, histidine decarboxylase gene.
tdc, tyrosine decarboxylase gene.