Table 1 Primer sequences used for amplifying Md-ACS1 and Md-ACO1 genes

Gene	Primer sequences	Amplicon size (bp)	Annealing temperature (℃)	Reference
Md-ACS1	Forward 5′ AGAGAGATGCCATTTTTGTTCGTAC 3′	1:489	58	Sunako et al (17)
	Reverse 5′ CCTACAAACTTGCGTGGGGATTATAAGTGT 3′
Md-ACO1	Forward 5′ TCCCCCCAATGCACCACTCCA 3′	1:525	65	Costa et al (5)
	Reverse 5′ GATTCCTTGGCCTTCATAGCTTC 3′