Table 4 Inhibitory effects of the 80% methanolic extract of Mongolian I. bungei and its n-hexane-, EtOAc-, n-BuOH-, and H2O-soluble portions against α-glucosidases
Samples | Inhibition (%) | IC50 (μg/mL) |
50 | 25 | 12.5 | 6.3 | 3.1 |
80% MeOH ext. | 90.9±1.7d1) | 55.8±1.4d | 22.0±1.1d | 11.0±1.2d | 4.9±0.3d | 21.7±1.3 |
n-Hexane layer | 97.0±1.0b | 82.8±2.4c | 38.9±2.1c | 13.6±2.1c | 6.9±0.5c | 14.3±0.6 |
EtOAc layer | 99.4±2.1a | 96.6±1.4a | 85.3±4.2a | 64.5±1.5a | 32.0±1.2a | 4.6±0.1 |
n-BuOH layer | 11.4±1.5f | 9.4±0.9e | 8.5±1.4e | 6.7±0.5e | 3.5±1.5e | >200 |
H2O layer | 92.4±2.5c | 85.9±1.2b | 74.8±2.0b | 44.4±0.6b | 27.0±2.1b | 6.7±0.4 |
Acarbose2) | 14.1±0.9e | 9.3±1.9e | 2.6±0.5f | 2.1±0.4f | 1.1±0.4f | 473.2±5.5 |
Scavenging activities are expressed as the mean±SD of triplicate experiments. Mean separation within columns of respective seasons by Duncan’s multiple range test at p=0.05.
Acarbose was used as a positive control.