Table 2 DPPH radical scavenging activity of 80% methanol extract of Mongolian I. bungei and its n-hexane, EtOAc-, n-BuOH-, and H2O-soluble fractions

Samples Inhibition (%) IC50 (μg/mL)
500 250 125 62.5 31.3
80% MeOH ext. 72.0±1.2c1) 58.1±0.2c 46.0±1.3c 31.2±1.3c 11.4±2.0bc 173.2±16.8
n-Hexane layer 61.8±1.7e 45.7±1.2e 28.5±0.8e 19.9±1.4d 10.4±1.8cd 310.4±11.1
EtOAc layer 80.2±1.1b 59.4±0.8b 48.2±1.3b 32.2±1.6b 14.3±0.2b 148.7±11.6
n-BuOH layer 40.2±0.2f 32.5±1.1f 24.3±1.5f 16.7±2.2e 9.8±0.8d >500
H2O layer 64.3±0.9d 52.7±1.2d 41.2±1.7d 34.4±1.5b 12.3±1.4b 217.6±12.6
(+)-Catechin2) 99.9±0.7a 99.8±0.4a 96.2±1.4a 87.6±1.1a 74.1±0.6a 15.0±0.9
Scavenging activities are expressed as the mean±SD of triplicate experiments. Mean separation within columns of respective seasons by Duncan’s multiple range test at p=0.05.
(+)-Catechin was used as a positive control.