Table 2 DPPH radical scavenging activity of 80% methanol extract of Mongolian I. bungei and its n-hexane, EtOAc-, n-BuOH-, and H2O-soluble fractions
| Samples | Inhibition (%) | IC50 (μg/mL) |
| 500 | 250 | 125 | 62.5 | 31.3 |
| 80% MeOH ext. | 72.0±1.2c1) | 58.1±0.2c | 46.0±1.3c | 31.2±1.3c | 11.4±2.0bc | 173.2±16.8 |
| n-Hexane layer | 61.8±1.7e | 45.7±1.2e | 28.5±0.8e | 19.9±1.4d | 10.4±1.8cd | 310.4±11.1 |
| EtOAc layer | 80.2±1.1b | 59.4±0.8b | 48.2±1.3b | 32.2±1.6b | 14.3±0.2b | 148.7±11.6 |
| n-BuOH layer | 40.2±0.2f | 32.5±1.1f | 24.3±1.5f | 16.7±2.2e | 9.8±0.8d | >500 |
| H2O layer | 64.3±0.9d | 52.7±1.2d | 41.2±1.7d | 34.4±1.5b | 12.3±1.4b | 217.6±12.6 |
| (+)-Catechin2) | 99.9±0.7a | 99.8±0.4a | 96.2±1.4a | 87.6±1.1a | 74.1±0.6a | 15.0±0.9 |
Scavenging activities are expressed as the mean±SD of triplicate experiments. Mean separation within columns of respective seasons by Duncan’s multiple range test at p=0.05.
(+)-Catechin was used as a positive control.