Table 3 Free amino acid content (ppm) of Doenjang fermented with Aspergillus oryzae SCF-6 and protease
Amino acids | Control | Protease A1) | Protease B | Protease A+B |
0 week | 8 week | 0 week | 8 week | 0 week | 8 week | 0 week | 8 week |
Histidine | 10.70±0.04 | 22.53±1.23 | 9.48±0.32 | 21.81±0.70 | 17.16±0.18 | 26.15±0.91 | 14.80±0.03 | 26.85±2.41 |
Serine | 17.53±0.49 | 41.72±1.70 | 18.53±0.63 | 40.26±0.48 | 22.79±0.16 | 44.30±0.35 | 22.64±0.39 | 44.64±5.46 |
Arginine | 17.72±1.03 | 18.81±0.78 | 21.44±0.84 | 22.28±0.15 | 55.73±2.78 | 30.80±0.33 | 47.82±0.10 | 28.75±3.09 |
Glycine | 13.03±0.13 | 33.71±0.85 | 12.77±0.75 | 32.18±0.71 | 16.48±0.81 | 38.55±0.72 | 15.54±0.40 | 37.49±3.49 |
Aspartic acid | 12.09±0.90 | 38.91±3.08 | 9.03±0.09 | 25.37±3.04 | 9.74±0.89 | 29.92±1.21 | 9.78±0.80 | 27.82±4.71 |
Glutamic acid | 84.12±5.74 | 137.32±8.42 | 69.69±0.08 | 96.76±9.36 | 71.53±4.75 | 100.58±3.15 | 79.57±4.32 | 98.33±12.67 |
Threonine | 14.67±0.13 | 30.77±1.14 | 14.61±0.16 | 15.76±12.68 | 19.82±0.11 | 35.17±0.43 | 18.72±0.25 | 36.58±4.93 |
Alanine | 27.19±1.61 | 40.87±1.35 | 23.66±0.19 | 33.23±2.30 | 23.50±1.25 | 35.11±0.18 | 25.53±0.71 | 33.72±3.68 |
Proline | 16.13±0.41 | 35.84±0.67 | 14.91±0.74 | 31.94±0.59 | 26.05±0.51 | 47.83±0.40 | 22.56±0.04 | 42.42±2.94 |
Cysteine | 4.07±0.53 | 0.17±0.02 | 4.73±0.18 | 0.20±0.07 | 4.44±0.12 | 0.27±0.01 | 5.16±0.23 | 0.33±0.07 |
Lysine | 42.60±2.53 | 53.77±2.56 | 34.98±2.21 | 33.76±5.63 | 40.85±4.09 | 33.61±0.38 | 45.03±2.25 | 30.10±4.13 |
Tryptophan | 32.67±0.78 | 44.04±0.28 | 31.92±1.25 | 48.88±3.07 | 45.02±1.50 | 40.95±1.28 | 42.81±1.23 | 46.42±5.08 |
Methionine | 11.19±0.24 | 10.01±0.51 | 11.88±0.02 | 11.08±0.47 | 13.94±0.26 | 11.10±0.55 | 15.11±0.05 | 12.32±1.37 |
Valine | 23.79±1.52 | 0.88±0.02 | 18.99±0.03 | 0.82±0.02 | 29.06±1.61 | 0.95±0.01 | 28.17±0.38 | 0.91±0.08 |
Isoleucine | 19.87±0.99 | 49.81±1.02 | 17.34±0.05 | 46.67±1.14 | 28.35±1.72 | 56.05±0.79 | 26.61±0.20 | 54.47±4.56 |
Lucine | 50.88±2.47 | 86.62±1.98 | 54.73±0.14 | 88.13±2.09 | 69.01±4.38 | 92.01±1.56 | 72.35±0.26 | 93.77±8.03 |
Phenylalanine | 47.51±1.16 | 75.48±0.62 | 51.68±1.95 | 85.66±5.35 | 68.33±0.47 | 92.39±3.36 | 65.18±2.23 | 100.62±9.79 |
Protease A isolated from Bacillus licheniformis, Protease B isolated from Aspergillus species, Protease A+B=0.5:0.5 ratio.