Table 2 Enzyme activity (unit/g) of Doenjang fermented with Aspergillus oryzae SCF-6 and protease
| Fermentation period (week) | F-value |
0 | 2 | 4 | 8 |
Alpha-amylase | Control | 0.06±0.00Cab2) | 0.06±0.02Ba | 0.05±0.02Cab | 0.03±0.01Cb | 0.141 |
Protease A1) | 0.06±0.02Ca | 0.06±0.01Ba | 0.05±0.00Cab | 0.03±0.00Cb | 0.011 |
Protease B | 0.41±0.01Ab | 0.42±0.03Ab | 0.52±0.02Aa | 0.16±0.01Ac | <0.0001 |
Protease A+B | 0.38±0.00Bb | 0.45±0.04Aa | 0.44±0.02Ba | 0.12±0.04Bc | <0.0001 |
F-value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | |
Protease | Control | 168.06±39.72Cb | 261.25±29.03Ca | 187.92±19.86Bb | 175.69±29.03Cb | 0.0191 |
Protease A | 643.19±44.31Ba | 641.67±64.17Aa | 574.44±51.94Aab | 533.19±44.31Bb | 0.0815 |
Protease B | 701.25±25.97ABa | 513.33±51.94Bbc | 569.86±13.75Ab | 481.25±29.03Bc | 0.0002 |
Protease A+B | 757.78±12.22Aa | 725.69±32.08Aab | 603.47±1.53Ac | 693.61±42.78Ab | 0.0007 |
F-value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | |
Protease A isolated from Bacillus licheniformis, Protease B isolated from Aspergillus species, Protease A+B=0.5:0.5 ratio.
Any means in the same fermentation time (A-B) or enzyme (a-d) followed by different letters are significantly (p<0.05) different by Duncan’s multiple range test.