Table 1
HPLC and HPLC/MS condition

	HPLC Condition
Instrument	Dionex system
Column	Inertsil ODS-2(C18) Column (4.6 mm×250 mm; GL Science, Tokyo, Japan)
Solvent	A: Deionised water
	B: 20% acetonitriles
	0-8 min increased 1-99% B
	18-29 min kept 99% B
	29-32 min decreased 99-1% B
	32-40 min kept 1% B
Column temperature	35℃
Flow rate	1 mL/min
Infection volume	20 μL
	Mass system
Polarity	ESI+
Capillary temperature	275
Voltage	Pray: 5 kV and capillary: 15 V
Mass scan range (m/z)	100-600