Table 2 Analysis of extraction efficient rate (%), sugar contents (°Brix), and acidity (pH) of blueber ry extract according to different extraction temperatures, extraction times, and shaking speeds after enzyme treatment

Variation1)	Conditions
	Extraction rate (%)	°Brix	pH
	Temperature (°C)
40	87.62±0.15^cb2)	1.20±0.06^bc	3.17±0.02^b
45	87.91±0.05^a	1.20±0.00^ab	3.23±0.02^a
50	87.58±0.11^c	1.30±0.06^a	3.19±0.00^b
55	87.65±0.05^b	1.10±0.00^c	3.23±0.01^a
Time (h)
0	85.21±0.05^c	1.20±0.00^b	3.23±0.00^a
2	86.82±0.28^ab	1.20±0.00^b	3.22±0.00^a
3	87.88±0.10^a	1.20±0.00^b	3.20±0.01^b
4	87.39±0.05^a	1.27±0.06^a	3.20±0.01^b
5	86.68±0.05^b	1.10±0.00^c	3.20±0.02^b
Agitation rate (rpm)
0	87.51±0.05^d	1.17±0.06^a	3.21±0.01^c
30	87.92±0.10^cd	1.17±0.06^a	3.22±0.01^b
60	88.48±0.79^bc	1.20±0.00^a	3.19±0.00^d
90	89.19±0.00^a	1.20±0.00^a	3.22±0.01^b
120	88.92±0.05^ab	1.07±0.06^b	3.25±0.01^a

0.1% cellulase:pectinase mix (1:1) was used as a fixed factor for temperature variation and treated for 3 h at 45°C. 0.1% cellulase:pectinase mix (1:1) and 45°C were fixed factors for extraction time variation. For agitation rate, 0.1% cellulase:pectinase mix (1:1) were treated at 45°C for 3 h.
Values represent the mean±SD (n=3) and different letters indicate significant differences (p<0.05).