Table 1 Extraction efficiency rate (%), acidity (pH) and sugar contents (°Brix) of blueberry were summarized based on different enzymes and its ratio

Enzyme ratio (%)1) Extraction rate (%) °Brix pH
Control 0.00 85.02±0.02i2) 1.13±0.06bc 3.27±0.01a
pectinase 0.05 87.06±0.06cd 1.17±0.06abc 3.24±0.01b
0.10 87.93±0.12ab 1.10±0.00c 3.28±0.01a
0.15 87.49±0.10bc 1.17±0.06abc 3.22±0.01cd
0.20 87.81±0.10ab 1.13±0.06bc 3.20±0.01efg
cellulase 0.05 85.94±0.16gh 1.20±0.00ab 3.22±0.01cd
0.10 86.18±0.18fg 1.20±0.00ab 3.23±0.00bc
0.15 85.72±0.38h 1.23±0.06a 3.22±0.01cde
0.20 86.55±0.51ef 1.20±0.00ab 3.22±0.01cd
cellulase: pectinase (1:1) 0.05 86.84±0.31de 1.10±0.00c 3.24±0.01b
0.10 87.77±0.30ab 1.17±0.06abc 3.21±0.01def
0.15 87.25±0.31cd 1.20±0.00ab 3.19±0.01g
0.20 88.14±0.32a 1.23±0.06a 3.20±0.01fg
Enzyme ratio (%) represents 67.5 U for 0.05%, 270 U for 0.10%, 607.5 U for 0.15%, 1,080 U for 0.20% in pectinase, while 60 U for 0.05%, 240 U for 0.10%, 540 U for 0.15%, 960 U for 0.20% in cellulase. 63.75 U for 0.05%, 255 U for 0.10%, 573.75 U for 0.15%, 1,020 U for 0.20% in cellulase:pectinase (1:1). Each treatment was incubated with enzymes for 3 h at 45°C.
Values represent the mean±SD (n=3) and different letters indicate significant differences (p<0.05).