Table 3 ABTS+ radical scavenging activity of the 80% methanolic extract of A. dioicus var. kamtschaticus roots and its n-hexane-, EtOAc-, n-BuOH-, and H2O-soluble por tions

Sample (μg/mL) Inhibition (%) IC50 (μg/mL)1)
200 100 50 25 12.5
80% MeOH extract 94.2±2.7 88.6±2.2 42.6±2.0 21.5±1.1 12.8±0.7 31.9±1.2
n-Hexane layer 10.3±0.7 8.2±0.5 5.3±0.3 3.9±0.2 1.8±0.2 >200
EtOAc layer 91.0±2.8 85.1±2.4 82.8±2.1 60.9±1.8 35.4±1.5 18.9±2.1
n-BuOH layer 68.6±2.3 40.4±2.1 21.0±0.7 8.4±0.5 2.7±0.2 51.0±2.9
H2O layer 1.7±0.1 1.4±0.2 1.1±0.2 0.5±0.1 0.3±0.1 >200
(+)-Catechin2) 99.9±2.3 99.9±2.0 71.8±1.8 28.4±1.1 8.9±0.8 34.9±2.1
All samples were examined in triplicate experiments. Inhibitory effects are expressed as the mean±SD of triplicate experiments.
(+)-Catechin was used as a positive control.