Table 3 ABTS+ radical scavenging activity of the 80% methanolic extract of A. dioicus var. kamtschaticus roots and its n-hexane-, EtOAc-, n-BuOH-, and H2O-soluble por tions
Sample (μg/mL) | Inhibition (%) | IC50 (μg/mL)1) |
200 | 100 | 50 | 25 | 12.5 |
80% MeOH extract | 94.2±2.7 | 88.6±2.2 | 42.6±2.0 | 21.5±1.1 | 12.8±0.7 | 31.9±1.2 |
n-Hexane layer | 10.3±0.7 | 8.2±0.5 | 5.3±0.3 | 3.9±0.2 | 1.8±0.2 | >200 |
EtOAc layer | 91.0±2.8 | 85.1±2.4 | 82.8±2.1 | 60.9±1.8 | 35.4±1.5 | 18.9±2.1 |
n-BuOH layer | 68.6±2.3 | 40.4±2.1 | 21.0±0.7 | 8.4±0.5 | 2.7±0.2 | 51.0±2.9 |
H2O layer | 1.7±0.1 | 1.4±0.2 | 1.1±0.2 | 0.5±0.1 | 0.3±0.1 | >200 |
(+)-Catechin2) | 99.9±2.3 | 99.9±2.0 | 71.8±1.8 | 28.4±1.1 | 8.9±0.8 | 34.9±2.1 |
All samples were examined in triplicate experiments. Inhibitory effects are expressed as the mean±SD of triplicate experiments.
(+)-Catechin was used as a positive control.