| Items | Conditions | ||
| Instrument | Waters Co., USA, 2695 | ||
| Column | waters, C18, 3.5 μm (4.6×150 mm) | ||
| Time (min) | A(%) (0.1%Trifluoroacetic acid) | B(%) (Acetonitrile) | |
| Mobilie phase | 0 | 95 | 5 |
| 1 | 95 | 5 | |
| 20 | 71 | 29 | |
| 21 | 71 | 29 | |
| Flow rate | 1 mL/min | ||
| Detector | UV detector | ||
| Injection volume | 20 μL | ||
| Wave length | 280 nm | ||