Table 1
HPLC conditions for analysis in trigonelline, caffeine and chlorogenic acid of coffee extracts
Items
Operating conditions
Instrument
ShimadzuProminence
Column
C
18
(4.6mm×150mm,5μm)
Columntemp.
40℃
Mobilephase
10mMHCl:MeOH=90:10
Injectionvolume
10μL
Flowrate
1.0mL/min
Detector
DiodeArraydetector